We started to look at how gut microbes interact with fats in the diet with a study of dietary soy bean oil and its effect on Lactobacilli in the small intestine (Di Rienzi, 2018). This led us to think more broadly about the fats produced by gut bacteria themselves, and we chose to focus on a particular class of lipids, sphingolipids, because only a restricted group of Bacteria (i.e., the Bacteroidetes) make these lipids in the gut (Heaver, 2018). Through genetic manipulation of a prominent Bacteroides species (B. thetaiotaomicron) and studies in mice, we showed that the capacity to produce sphingolipid in the gut affect levels of sphingolipid (ceramides) in the liver (Johnson, 2020), which can be relevant for metabolic disease. We are now delving into other organ systems to assess how production of sphingolipids by gut bacteria affects the host sphingolipodome.